Talaga & Sytnik 2002

Talaga, D. S.; Jia, Y.; Bopp, M. A.; Sytnik, A.; DeGrado, W. A.; Cogdell, R. J.; Hochstrasser, R. M.  “Single-molecule dynamics associated with protein folding and deformations of light-harvesting complexes” Springer Series in Chemical Physics (Single Molecule Spectroscopy: Nobel Conference Lectures), (2002), 67, 313-325.

Two new applications of single molecule methods in biology are described. In one, single assemblies of the intact light harvesting complex LH2 from Rhodopseudomonas acidophila were bound to mica surfaces at 300K and examined by observing their fluorescence after polarized light excitation. They mostly behaved as electrically elliptic absorbers whose ellipticity fluctuates, showing that there is a mobile structural deformation. The other application involves the folding and unfolding of a coiled coil GCN4-P1 peptides. By following the trajectory of individual members of a folding ensemble we are able to evaluate of men and distributions of properties not available from bulk studies. 

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